Expressions of related genes in the shell of accumbens nuclei when constructing a rat model of chronic morphine-induced conditioned place aversion

doi:10.3969/j.issn.2095-4344.2013.20.011

Abstract

Abstract:

BACKGROUND: Adenylate cyclase Ⅷ is involved in the promotion of morphine tolerance, withdrawal and enhancement, and plays an important role in plastic changes, such as the advanced long-term enhancement effect, long-term memory and stress adaptation.
OBJECTIVE: To explore the changes of adenylate cyclase Ⅷ gene expression in the shell of accumbens nuclei before and after development of chronic morphine-induced conditioned place aversion in a rat through naloxone reminder addiction withdrawal.
METHODS: Clean grade Sprague Dawley rats were divided into three groups: morphine+naloxone group, morphine+saline group and saline+naloxone group. Rats in the former one group received intraperitoneal injection of 10 mg/kg morphine continuously for 6.5 days, and intraperitoneal injection of 0.3 mg/kg naloxone; then the conditioned place aversion model was established combined with the conditioned place training. Rats in the latter two groups were injected with the same dose of saline as the morphine+naloxone group. The adenylate cyclase Ⅷ gene expression in the shell of accumbens nuclei was detected with immunohistochemistry method before and after development of chronic morphine-induced conditioned place aversion model.
RESULTS AND CONCLUSION: Before chronic morphine-induced conditioned place aversion model establishment, there was no significant difference of the adenylate cyclase Ⅷ gene expression in the shell of accumbens nuclei (F=4.651, P=0.052); after conditioned place aversion establishement, the adenylate cyclase Ⅷ gene expression in the shell of accumbens nuclei in the morphine+naloxone group was significantly higher than that in the morphine+saline group and saline+naloxone group (F=4.874, P=0.028). The results indicate that the changes of adenylate cyclase Ⅷ gene expression may be one of the important molecular underpinnings of the conditioned place aversion.

Key words: tissue construction, experimental modeling in tissue construction, morphine, withdrawal, conditioned place aversion, adenylate cyclase Ⅷ, animal model, shell of accumbens nucleus, National Natural Science Foundation of China

CLC Number:

R318

Zhang Jing-dan, Li Wen-qiang, Song Xiu-hua, Lou Bai-yu, Shi Yu-zhong, Li Yi. Expressions of related genes in the shell of accumbens nuclei when constructing a rat model of chronic morphine-induced conditioned place aversion [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(20): 3687-3691.

Figures/Tables 2

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